We have developed a low-cost high throughput method to quantify the expression of these miRNAs via classic total RNA extraction without interference from genomic DNA. More than 150 human and more than 100 mouse miRNAs have been validated using as little as 100ng total RNA per miRNA. This method provides accurate sensitive miRNA expression profiles which can be used to identify specific miRNA signatures associated with tissues, biological processes or diseases.
Authors: JF Laes, P Lienard, C Sequaris, I Gonze and JP Detiffe
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