Many high content analysis (HCA) platforms offer limited capability for primary screening due to their low throughput, large data files and incompatibility with high-density microplates, making it impractical to deploy such systems for high-throughput screening. Laser-scanning microplate cytometers, such as the Acumen Explorer, offer rapid read and analysis times (typically <10 minutes). In addition, 96, 384 and 1,536 well microplates can be analysed at equivalent plate read times since scanning is performed on an area and not well basis.

For example, in measuring protein kinase activation (a common high content assay), the multi-step nature of the protocol is complex and requires much coordinated labour to perform. Here, we model the integration of the Protedyne BioCube System and the Acumen Explorer for practical high-throughput, high content screening of protein kinases. Integration of cell culture, compound addition, immunodetection and analysis results in capacities up to 40,000 wells/day, with superior process control.

Authors: Wayne Bowen, Jason Quarles and Michael Catalano

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