Task

Preparation and processing of microarrays for DNA, peptide, antibody, and carbohydrate applications

Problem
To obtain data of highest quality and meaningfulness a careful optimisation of protocols is necessary. Experimental errors need
to be reduced as much as possible.

Solution
We have developed a workflow for optimisation of array preparation and for processing protocols. Only 4 independent model experiments and solvent, immobilisation and blocking conditions. Optimal conditions or high quality microarray experiments were determined and used for array production and slide processing at the FGCZ.
Authors: Jens Sobek, Catharine Aquino and Ralph Schlapbach

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